Starting with Genefinder
Genefinder is available in two forms, a text based program
genefinder and one with a graphical display xgenefinder.
Both should be in your search path, as they are located in the
directory /usr/local/bin. The difficult part with genefinder is using
the organism specific tables that help define gene positions. The
distribution comes with four, and we have an additional Arabidopsis
set from the European Sequencing group. The table name files are
located in the directory /usr/local/lib/genefinder and are named as
follows
- humtables (human)
- nemtables (nematode)
- thaletables (arabidopsis)
- thaltables (arabidopsis)
- wormtables (same as nemtables)
If you have a DNA sequence in fasta format with the name
"mysequence.seq" and you wish to run xgenefinder with the nematode
tables, you may run with a command similar to the following;
xgenefinder -tablenamefile /usr/local/lib/genefinder/nemtables mysequence.seq
You need to include the full path to the table name file,
therefore if you always want to run using a particular set of tables,
you can save yourself some typing by creating a file call
.genefinderrc in your home directory with the line:
tablenamefile: /usr/local/lib/genefinder/nemtables
Addional notes on setting parameters and the .genefinderrc file are available in the file Humangenefinding.html
The main documentation file is genefinder.doc.